The University of Vermont

Cooperative  for Real Education in Agricultural Management

protocols Protocols

The following protocols were written by CREAMers to aid in future CREAMer's understanding of certain issues. Some topics are only briefly covered and further research may be necessary.

General Health Protocol
Mastitis Protocol
Heat Detection Protocol
Cow Evaluation Protocol
Mating Protocol
Calving Protocol
Fresh Cow Protocol
Feeding Protocol

General Health Protocol
 

Hoof Rot
Symptoms: inflammation, redness, swelling, scabbing, limping 
Prevention: Make cows walk through a Lincomix, Copper Sulfate, or Tetracycline foot bath when going out in and coming back from the dry lot. Change the bath every three days. 
Treatment: Clean the hoof thoroughly, removing any debris. Apply Kopertox/Hoof Tect to affected hooves with a narrow paint brush until fully healed. 

 
Udder Rot 
Symptoms: smelly, crusty, rotten, scabby udders 
Treatment: Treatment: Pick scabs off udder, making sure to get to the skin area, to ensure proper healing. Some bleeding may occur. Now spray vinegar and copper sulfate (blue liquid) or apply Derma Clens to the affected area. 

 
Calf Scours
Symptoms: soft, watery, smelly, or yellow diarrhea
Treatment: Nutra Lyte or Resorb.

 
Chapped Teats
Symptoms: dry, red, chapped, or irritated teats 
Treatment:  Wash the affected parts of the teat with warm water and soap after each milking, then dry with a clean cloth. Gently apply red udder ointment. Be sure to wash the udder and teats thoroughly before the next milking to avoid contamination of the milk. 

 
Hard Quarter (Edema)
Symptoms:  a slow milking quarter that is hard and swollen 
Treatment:  Massage approximately 10 ml of white uttermint onto the quarter 2-3 times per day after the first milking & stripping out. Repeat for 2-4 days. 

 
Wounds
Treatment: Clean affected area and apply RV wound powder, Derma Clens, or Granulex spray. 

 
Minor Sprains or Bruises
Symptoms: limping or a discoloration where sore
Treatment: Apply white linement to injured area.

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Mastitis Protocol

Mastitis is an infection of the mammary gland. It is important to treat quickly to prevent mammary tissue damage, which leads to decreased milk production. There are two forms of mastitis: sub-clinical and clinical, the former being difficult to detect.

General Information

  • aseptically sample all four quarters (see sampling procedure)
  • strip all infected quarters until chunks are gone
  • the rest of the milk can go into the bulk tank when the chunks are gone
  • milk the infected cow last until you receive a clean sample from the lab or until she is dried off
  • if a milker has been on a cow with mastitis, soak the inflations (claw upside down) in a bucket of warm water and iodine for about five minutes before it is used on another cow
Sub-Clinical Mastitis

Symptoms:  a milk sample test reporting a high somatic cell count (>1 million cells/mL), edema

Treatment:

  • milk sub-clinical cases last to avoid infecting other cows
  • apply uddermint to quarters suffering from edema (see general health protocol)
  • make sure cows with sub-clinical infects are milked out completely, as residual milk provides a great atmosphere for microbial growth
  • if chuncks begin to reappear, follow the procedures for clinical mastitis
  • sample when you think the infection is clearing up
  • the cow no longer needs to be milked last once a clean sample comes back from the lab
Clinical Mastitis

Symptoms:  flakes or chunks of somatic cells, edema, and a high somatic cell count

Treatment:

  • strip out infected quarters completely at each check, then dip the teat in iodine
  • make sure infected quarters are milked out completely at chores
  • if one quarter appears to be milked out before the others, strip out that quarter again and put the milker back on, as there could be a chunk blocking the milk
  • the infection has become sub-clinical when chunks and flakes are no longer seen after stripping (see sub-clinical treatment procedures)
  • apply uddermint to hard quarters
  • lab results will tell whether antibiotics should be used to treat the infection
  • milk from cows treated with antibiotics does not go into the bulk tank until they test okay (tube turns yellow) on a Delvo test
Aseptic Sampling Procedure
  1. After the cow has been stripped and dipped, wipe all dirt, dust, feces, etc. from teat ends with alcohol soaked cotton balls. Start with the teats furthest from you and work toward the two closest to you, so you do not recontaminate the teats with your arm.
  2. Beginning with the teat closest to you, strip milk into a pre-labeled sampling tube, filling it about 1/3 of the way. Do not touch the teat end or allow any milk that may have touched your hand into the tube.
  3. Repeat this procedure for all quarters, working your way back to the ones furthest from you.
  4. Tubes should be labeled with the cow's number and the quarter from which the sample was taken. Do this with a permanent marker.
  5. Place all tubes in a rack and put the rack into UVM's refrigerator. Leave the properly filled out paper work withy the rack of tubes.
Delvo Testing Procedure
  1. Wash your hands thoroughly with soap and water.
  2. Place one ampule (tiny test tube) in the Delvo test box for each milk sample.
  3. Place one nutrient tablet into the purple agar medium in each ampule.
  4. Place a dry sample testing pipette on the dosage syringe.
  5. Push in the piston rod all the way, bringing the tip of the pipette approximately one centimeter into the milk sample and letting the piston rod return slowly to its original position, bringing some milk into the pipette.
  6. Transfer the sample from the pipette (approximately .1 mL) completely into the ampule.
  7. Use a new disposable pipette for each milk sample.
  8. Incubate ampules in the provided block heater for 2.5 hours (+/- 5 minutes).
  9. If the lower 2/3 of the agar medium is yellow, the milk is absent of antibiotic residues, and milk from these cows can go into the bulk tank.
  10. A purple color indicates the presence of antibiotics above the detection limit, and milk from these cows can not go into the bulk tank.
  11. A partly yellow, purple color indicates presence in a concentration near the detection limit.
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Heat Detection Protocol

General Information

  • Cows ovulate or come into heat, about every 21 days. They display certain signals 10-12 hours before ovulation occurs.
                      Signs of heat:
    •   standing for another cow to mount or ride
    •   clear, stringy discharge from the vulva
    •   trying to mount or ride one or several cows
    •   bawling
    •   redness or swelling of the vulva
    •   restlessness, head butting
  • Head butting and restlessness do not always indicate a heat. Observe individual cows and their heat signs in order to know which signs are significant.
  • It is extremely important to spend 20 minutes observing the cows when they are turned out in the pen. Be sure to look at the breeding wheel before you watch heats so you know which cows are due to be in heat on a certain day.
  • Fresh heifers should not be bred any earlier than 60 days.
  • If a cow comes into heat and is due to be bred, mark it in the herd book. The sheet on the gestation tabulator wheel should tell you who the sire will be for that cow. Leave a message on Don's board on his office door with the cow's number and the sire's name or contact him in person.
  • If a cow comes into heat many times in a one day period, she may be cystic and should be looked at by a veterinarian.
Breeding Wheel Operation
  • If a heat is noticed and is not pegged on the breeding wheel, place the appropriate colored peg on that day.
Peg Color:
0 - 60 days  yellow
60 - 90 days green
90 - 120 days  red
120 days +  black
  • The calendar underneath the wheel indicates the number that the wheel should be at on a particular day of the month.
  • The wheel should be turned at each morning chore.
  • A cow that should be coming into heat is indicated by the peg next to #21 and it says heat, in blue.
  • Cow pegs are placed on the wheel until the cow is confirmed pregnant, then the peg should be moved to the Gestation Tabulator.
Gestation Tabulator

It is used as a tool to keep track of where cows are in their gestation cycle. It also indicates when to dry off a cow or when it is due to freshen.

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Cow Evaluation Protocol

Begin with the cow's pedigree. This will give information such as classification score, TPI, PTA's for fat and protein, and transmitting ability and reliability. Another area of interest in evaluating a cow is the linear traits or characteristics. These trait descriptions include: milking speed, disposition, stature, dairy form, strength, body depth, rump width, rump angle, legs rear view, legs side view, foot angle, rear udder height, rear udder width, udder support, udder depth, front teat placement, and front teat length.

The following information is included on an animal's pedigree:

  • Animal Identification Information - registration name, type production index, classification, and date of birth
  • Predicted Transmitting Abilities (PTA) - PTA's for milk, fat, type, udder composite, and reliability
  • Sire's Identification Information - line one includes registered name and TPI values, line two provides registration number, nation code, and recessive tests, and line three provides age at classification and final score
  • Sire's Type Production Index (TPI) - compares PTA for protein, fat, type, and udder composite to rank sires on their ability to transmit a balance of these four traits
  • Udder Composite Index (UDC) - combines linear type trait information on six udder traits into a single number value
  • Sire Summary for Production and Type Traits - line one includes PTA's for milk, protein, fat, type, and reliability, line two includes PTA$ for cheese yield dollars, fat, and protein, line three includes PTA%, and line four includes PTA's for type, udder composite, and percent reliability.
  • Dam's Identification Information - line one includes the animal's registered name, line two includes the registration number, age at classification, final score, and major classification categories
  • Cow Type Production Index (PTI)
  • Predicted Transmitting Ability (PTA) - line one shows the cow's predicted transmitting ability for milk, fat, protein, and reliability, line two provides PTA$ for cheese yield, fat, and protein, line three includes PTA% for fat and protein, and line four includes PTA type
  • Dam's Production Records - line on includes information on the type of DHI testing program, age at calving, number of times milked per day, length of record in days, pounds of milk, fat percent, pounds of fat, protein percent, and pounds of protein, line two includes the length of complete record in days, pounds of milk, fat percent, pounds of fat, protein percent, and pounds of protein
  • Lifetime Production Records - once a cow produces more than 100,000 pounds of milk in her lifetime, milk production (Life) will appear on the pedigree
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Mating Protocol

Breeding in the dairy industry is an important aspect in trying to remain competitive in the farming world. Careful thought and consideration must be taken in selecting bulls. CREAM's main goal in breeding is to breed for the most "well rounded" cow. Mating is the act of choosing the correct sire for any given cow based upon her genetic strengths and weaknesses. Its purpose is to produce a calf that is genetically superior to its dam. When choosing a mate for a cow, the following steps should be followed.

  1. Evaluate the cow through observation and her pedigree. Identify her strengths and weaknesses.
  1. Information on proven bulls can be found using bull search on the computer in Doug's office and in CREAM's meeting room. Information can also be found by looking at bulls in the red book (which contains all proven bulls of every stud) or by looking at individual sire study's sire directories. Evaluate bulls purchased and available for use. Identify their strong traits.
  1. Select a sire with traits that would best compliment and improve upon the cow's traits.
    • Selecting Sires Based on Linear Traits Linear traits are those such as body depth, udder support, and foot angle. Some of them are based on measurements while others are more subjective. Using linear traits, a sire with a trait superior to that of the cow is selected.
    •  Selecting Sires Based on aAa Numbers AAa numbers combine linear traits that often occur together in a cow. These combined traits are given names and corresponding numbers. Each cow or bull is assigned three aAa numbers. Cow's numbers are put together so that the first number in the set represents the quality she lacks and therefore needs the most. The second number is the second weakest and so on. Bull's aAa numbers are put together so that the first number represents the quality he is strongest in. Using aAa numbers, a sire is selected by matching the cow's numbers as closely as possible with a bull's numbers.
  1. Once a mate has been chosen for a cow, double check that he has all the desired traits identified upon cow evaluation. Also make sure that he does not posses any undesirable traits or that the two are related.
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Calving Protocol

Calving is approaching when the cow:

  • is restless, circling in stall
  • is breathing heavily
  • has a bloody discharge
  • is leaking milk
  • has loose tendons around tail head and between hooks and pins
During calving, the cow is either laying down or standing. Then the water bag breaks, and she is in labor (labored breathing, regular contractions). A calf may be visible. Normal presentation comes with nose and front feet first.

If the calf is too large, pulling may be necessary:

  • attach chains to the calf's front feet, just above the dewclaws
  • pull only when cow pushes to prevent tearing of uterus
  • pull with steady pressure so calf won't slip back in
If the presentation is abnormal:
  • sanitize your hands with Povidone solution, which is in the cabinet under the coffee maker in UVM's milk house
  • reach into cow's vagina and try to ascertain calf's position
  • call Doug if the calf's position is abnormal
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Fresh Cow Protocol

Once a cow has calved, reference the following steps.

  1. Offer large amounts of fresh warm water.
  1. Provide a lot of good quality, fresh TMR and hay. This should be monitored closely, as it is important that the cow is eating to maintain health after calving.
  1. Be sure that the calf appears healthy and safe.
  1. Administer 4 cc of oxytocin intra muscularly (IM) to the fresh cow right after parturition.
  1. Watch for the placenta, which should pass within 24 hours. If it has not passed by that time, alert the Student Herd Advisor, Doug, Don, or a veterinarian, as a retained placenta may cause serious medical problems.
  1. The fresh cow should be observed for any discomfort or abnormality. Things to look for are:  open mouth breathing
    • sunken eyes
    • lethargy
    • staggering
    • not eating
  1. The cow's temperature should be taken for ten days if anything out of the ordinary is observed. A fever is present when the cow's temperature is > 103 degrees or when a heifer's temperature is > 102.5 degrees.
  1. The cow is moved into the CREAM barn from the MAT barn at the next milking. Her colostrum should be fed to the calf. She should be milked into a bucket, not into the bulk tank.
  1. An udder swollen with edema is often seen in fresh cows. Refer to General Health Protocol.
  1. Fresh cows are used to being in the pasture with little human contact so be patient with cows needing extra time to adjust to being milked.
  1. Feed the cow the same ration as the other cows in the barn. Supplement the TMR with hay for the first week of milking.
  1. When heifers freshen, there is often chafing between her udder and her leg. She may be washed with warm water and iodine and treated with red udder ointment until healed if this occurs.
  1. For the first two months of lactation the cow will be at her maximum milk production and in a negative energy balance, requiring her to have access to a lot of good quality feed.
  1. The most common fresh cow problems to be aware of are:
    • ketosis
    • milk fever
    • edema and chafing
    • displaced abomasum
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Feeding Protocol

TMR

A mixture of silages and concentrates that are balanced to meet the nutritional requirements of the herd.

Lactation Information

  • Ideally, rations should be formulated to meet the requirements of each period in a lactation cycle. This is the best way to optimize milk yield. Currently, that is not available to the CREAM program so one ration is used for every cow.
  • In the first 2 - 4 months after calving, it is difficult to provide adequate nutrition to the cow because her milk yield is high and her feed intake is limited. Cows are in a negative energy balance and usually lose body weight during the months of heavy milk production.
  • At 2-3 months into the lactation period, daily milk production hits its peak and then starts to decline. It is at this point that body weight begins to increase again.
  • In addition, you ideal want a cow to have fat stores prior to calving. If she has stored fat, this helps to counter the negative energy balance created by high milk production that occurs shortly after calving.
Feeding Guidelines

The feeding program should be reevaluated once a month around the DHIA testing results.

There are two criteria for feeding: milk production results from DHIA and days in milk (lactation period)

The Feeding Program

Which cows get plus signs indication that they get a little more feed at chores?

  1. cows that are in the first 12 weeks of milk production
  2. high producers that are giving 70 or more pounds of milk at the time of the DHIA test results
Which cows get minus signs indicating that they get a little less feed at chores?
  1. cows that are beyond 12 weeks in milk
  2. lower producers that are giving less than 70 pounds of milk at the time of DHIA test results
The key to insuring that a good feeding program fulfills its goals is to push up the feed as many times in a day as possible. Whenever a group member is in the barn other than for chores or checks, he/she should push up the feed in front of all cows. No cow should have an empty plate!

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Last modified March 24 2003 09:22 AM

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