BIOTECHNOLOGY - definitions/descriptions

anticodon - complementary base sequence to codon on tRNA molecule e.g. CGA on alanine tRNA

bases - purines and pyrimidines which pair in DNA/RNA structure

base pairs - ADENINE and THYMINE (URACIL); GUANINE and CYTOSINE

codon - sequence of three bases on mRNA coding for a particuluar amino acid

DNA - deoxyribonucleic acid

exon - a sequence of bases in a gene which are part of the code for a sequence of amino-acids ( see introns)

intron - a sequence of bases in a gene which are nor part of the code for aminoacids are will be excised during transcription

mRNA - messenger RNA (transfers genetic code from nucleus to ribosome)

nucleotide - base + sugar + phosphate

plasmid - small piece of circular double stranded DNA found in bacteria and frequently carrying antibiotic resistance markers. Readily transferred between closely related bacteria

polymerases - enzymes involved in nucleic acid replication (either DNA or RNA )from a template

purines - adenine, guanine

pyrimidines - cytosine, thymine (DNA only), uracil (RNA only)

replication - synthesis of new DNA strands

restriction endonuclease - enzyme cutting a piece of DNA at a particular base endonuclease sequence

reverse transcriptase - an enzyme that converts RNA into DNA 

ribosome - site of protein synthesis

RNA - ribonucleic acid

"sticky ends"- pieces of single stranded DNA left at end of DNA molecule after cutting with a restriction endonuclease

transcription - synthesis of mRNA from DNA template

translation - synthesis of protein from mRNA template

tRNA - transfer RNA (adds individual amino acid to mRNA)

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